Identification of hitherto undefined B-cell epitopes by antibodies in the sera of vitiligo patients using phage-display Peptide library.
Identifieur interne : 003212 ( Main/Exploration ); précédent : 003211; suivant : 003213Identification of hitherto undefined B-cell epitopes by antibodies in the sera of vitiligo patients using phage-display Peptide library.
Auteurs : Zohreh Jadali [Iran] ; Mohammad Bagher Eslami ; Mohammad Hossein Sanati ; Parvin Mansoori ; Mahmoud Mahmoudi ; Nader Maghsoodi ; Mehdi Arbabi GhahroudiSource :
- Iranian journal of allergy, asthma, and immunology [ 1735-1502 ] ; 2003.
Abstract
A random 12 mers phage library was used to screen a pool of immunoglobulin fractions obtained from vitiligo patients. Subsequent to panning experiments, a panel of affinity selected phage from vitiligo patients were obtained. This panel was tested using an ELISA for their reactivity with pooled sera from patients and normal controls. Among the 16 randomly selected clones, two of clones showed distinct positive reactivity with the patient's sera compared with controls. The peptides displayed by these phages expressed the following amino acid sequences: SHMPLANQYQWA and NHVQAWEQFWDS. Thus, screening with phagedisplayed random peptide library of vitiligo sera can reveal peptide sequences that mimic vitiligo-related self-antigen.
DOI: 02.04/ijaai.197201
PubMed: 17301381
Affiliations:
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Zjadali@razi.tums.ac.ir.</nlm:affiliation><country wicri:rule="url">Iran</country></affiliation></author><author><name sortKey="Eslami, Mohammad Bagher" sort="Eslami, Mohammad Bagher" uniqKey="Eslami M" first="Mohammad Bagher" last="Eslami">Mohammad Bagher Eslami</name></author><author><name sortKey="Sanati, Mohammad Hossein" sort="Sanati, Mohammad Hossein" uniqKey="Sanati M" first="Mohammad Hossein" last="Sanati">Mohammad Hossein Sanati</name></author><author><name sortKey="Mansoori, Parvin" sort="Mansoori, Parvin" uniqKey="Mansoori P" first="Parvin" last="Mansoori">Parvin Mansoori</name></author><author><name sortKey="Mahmoudi, Mahmoud" sort="Mahmoudi, Mahmoud" uniqKey="Mahmoudi M" first="Mahmoud" last="Mahmoudi">Mahmoud Mahmoudi</name></author><author><name sortKey="Maghsoodi, Nader" sort="Maghsoodi, Nader" uniqKey="Maghsoodi N" first="Nader" last="Maghsoodi">Nader Maghsoodi</name></author><author><name sortKey="Arbabi Ghahroudi, Mehdi" sort="Arbabi Ghahroudi, Mehdi" uniqKey="Arbabi Ghahroudi M" first="Mehdi" last="Arbabi Ghahroudi">Mehdi Arbabi Ghahroudi</name></author></analytic><series><title level="j">Iranian journal of allergy, asthma, and immunology</title><idno type="ISSN">1735-1502</idno><imprint><date when="2003" type="published">2003</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">A random 12 mers phage library was used to screen a pool of immunoglobulin fractions obtained from vitiligo patients. Subsequent to panning experiments, a panel of affinity selected phage from vitiligo patients were obtained. This panel was tested using an ELISA for their reactivity with pooled sera from patients and normal controls. Among the 16 randomly selected clones, two of clones showed distinct positive reactivity with the patient's sera compared with controls. The peptides displayed by these phages expressed the following amino acid sequences: SHMPLANQYQWA and NHVQAWEQFWDS. Thus, screening with phagedisplayed random peptide library of vitiligo sera can reveal peptide sequences that mimic vitiligo-related self-antigen.</div></front></TEI><affiliations><list><country><li>Iran</li></country></list><tree><noCountry><name sortKey="Arbabi Ghahroudi, Mehdi" sort="Arbabi Ghahroudi, Mehdi" uniqKey="Arbabi Ghahroudi M" first="Mehdi" last="Arbabi Ghahroudi">Mehdi Arbabi Ghahroudi</name><name sortKey="Eslami, Mohammad Bagher" sort="Eslami, Mohammad Bagher" uniqKey="Eslami M" first="Mohammad Bagher" last="Eslami">Mohammad Bagher Eslami</name><name sortKey="Maghsoodi, Nader" sort="Maghsoodi, Nader" uniqKey="Maghsoodi N" first="Nader" last="Maghsoodi">Nader Maghsoodi</name><name sortKey="Mahmoudi, Mahmoud" sort="Mahmoudi, Mahmoud" uniqKey="Mahmoudi M" first="Mahmoud" last="Mahmoudi">Mahmoud Mahmoudi</name><name sortKey="Mansoori, Parvin" sort="Mansoori, Parvin" uniqKey="Mansoori P" first="Parvin" last="Mansoori">Parvin Mansoori</name><name sortKey="Sanati, Mohammad Hossein" sort="Sanati, Mohammad Hossein" uniqKey="Sanati M" first="Mohammad Hossein" last="Sanati">Mohammad Hossein Sanati</name></noCountry><country name="Iran"><noRegion><name sortKey="Jadali, Zohreh" sort="Jadali, Zohreh" uniqKey="Jadali Z" first="Zohreh" last="Jadali">Zohreh Jadali</name></noRegion></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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